Additional Selective and Differential Cell Culture Media
NEOGEN Columbia Blood Agar Base, 500 g
Columbia Blood Agar Base is used with blood for the isolation and cultivation of a wide variety of fastidious microorganisms. Columbia Blood Agar Base is not intended for use in the diagnosis of disease or other conditions in humans. A modification of the original Columbia Agar base formulation, Columbia Blood Agar Base provides a medium that is suitable for use with both defibrinated horse or sheep blood. The peptone mixture provides a source of nitrogen, essential vitamins and amino acids. The starch provides a carbon source and sodium chloride maintains osmotic balance. Originally described as a general purpose nutritious agar base by Ellner et al. Columbia Agar is more frequently used when enriched by the addition of sterile blood. The medium is suitable for supporting the growth and determining hemolytic reactions of a variety of microorganisms. For more information visit https://www.neogen.com/solutions/Microbiology/columbia-blood-agar-base
NEOGEN Modified Semi-Solid Rappaport-Vassiliadis (MSRV) Agar, 10 kg
Modified Semi-Solid Rappaport-Vassiliadis (MSRV) Agar is used with Novobiocin for the rapid detection of motile Salmonella spp. and is not intended for use in the diagnosis of disease or other conditions in humans. MSRV was developed in 1986 by De Smedt, Bolderdijk and Rappold as a rapid means of Salmonella detection. The medium, based upon Rappaport Vassiliadis broth, is inoculated directly from the preenrichment medium, in the center of the plate. Motile organisms spread from the center in the semi-solid agar, but non-salmonellas are inhibited by the selective agents. After overnight incubation the use of polyvalent salmonella antisera or a latex kit can confirm the presence of a Salmonella.For more information visit https://www.neogen.com/solutions/Microbiology/modified-semi-solid-rappaport-vassiliadis-agar
NEOGEN Phosphate Buffer, pH 7.2, 5 kg
Phosphate Buffer, pH 7.2 is used for the preparation of microbiological dilution blanks in a laboratory setting. Phosphate Buffer, pH 7.2 is not intended for use in the diagnosis of disease or other conditions in humans. The formula for phosphate buffer is specified by the American Public Health Association (APHA) for use in diluting test samples. Phosphate Buffer, pH 7.2 is specified for use in diluting water, dairy products and food for microbiological methods. In the examination of water and dairy products the addition of magnesium chloride is recommended. This buffer is also referred to as Butterfield’s Buffered Phosphate Diluent and recommended for examination of food. Phosphate Buffer, pH 7.2 stabilizes the pH of water used for dilutions.For more information visit https://www.neogen.com/solutions/Microbiology/phosphate-buffer-ph-72
NEOGEN Potato Dextrose Agar, 5 kg
Potato Dextrose Agar is used for the preparation and maintenance of fungal test strains used in the growth promotion test, suitability of the counting methods and negative controls as described in the Harmonized USP/EP/JP. Potato Dextrose Agar is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the Harmonized USP/EP/JP for the cultivation of fungi and specifically for the preparation of the Aspergillus brasiliensis test strain. Conforms to USP/EP/JP performance specification. The medium is commonly abbreviated to PDA. The extract from potato and dextrose provide a nutritionally rich base that encourages mold sporulation and pigment production. For more information visit https://www.neogen.com/solutions/Microbiology/potato-dextrose-agar
NEOGEN Tryptone No. 1, 10 kg
Tryptone No. 1 is an enzymatic digest of casein and meat for use in preparing microbiological culture media in a laboratory setting. Tryptone No. 1 is not intended for use in the diagnosis of disease or other conditions in humans. Tryptone No. 1 is an enzymatic digest of casein and meat used as a nitrogen source in culture media. Casein is the main protein of milk, and a rich source of amino-acid nitrogen. Tryptone No. 1 is rich in tryptophane, making it valuable for use in detecting indole production. The absence of detectable levels of carbohydrates in Tryptone makes it a suitable peptone in differentiating bacteria on the basis of their ability to ferment various carbohydrates. Several media containing Tryptone are specified in standard methods for multiple applications.For more information visit https://www.neogen.com/solutions/Microbiology/tryptone-number-1
NEOGEN EC Medium, Modified with Novobiocin, 500 g
EC Medium, Modified with Novobiocin is used for the selective enrichment of Escherichia coli O157:H7 in a laboratory setting. EC Medium, Modified with Novobiocin is not intended for use in the diagnosis of disease or other conditions in humans.EC Medium was developed by Hajna and Perry in an effort to improve the methods for the detection of the coliform group and E. coli. This medium consists of a buffered lactose broth with the addition of 0.15% Bile Salts Mixture. Growth of spore-forming bacteria and fecal streptococci were inhibited by the bile salts.EC Medium, Modified with the addition of Novobiocin was first described by Okrend and Rose. Okrend and Rose modified EC Medium by reducing the Bile Salts Mixture concentration to 1.12% and adding 20 mg/L of sodium novobiocin.For more information visit https://www.neogen.com/solutions/Microbiology/ec-medium-modified-with-novobiocin
NEOGEN PALCAM Broth, 500 g
PALCAM Broth is used with supplements for the selective enrichment of Listeria spp. from foods and environmental samples in a laboratory setting. PALCAM Broth is not intended for use in the diagnosis of disease or other conditions in humans. Listeria monocytogenes, described in 1926 by Murray, Webb, and Swann, is a widespread problem in food industries. Evidence from outbreaks of listeriosis indicate the principle route of transmission is via the consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. PALCAM Broth is based on the formulation of Van Netten et al. while he was investigating the isolation of Listeria spp. from food samples. PALCAM Broth has been used for testing Listeria from raw milk.For more information visit https://www.neogen.com/solutions/Microbiology/palcam-broth
NEOGEN Acuferm Peptone, 500 g
For more information visit https://www.neogen.com/solutions/Microbiology/acuferm-peptone
NEOGEN Fastidious Anaerobe Broth, 500 g
Fastidious Anaerobe Agar is used for the cultivation of anaerobic microorganisms and is not intended for use in the diagnosis of disease or other conditions in humans.A primary isolation medium capable of growing most clinically significant anaerobes. Developed by Lab M (Neogen Corporation), comparisons have shown this medium to be superior to other formulations as a primary isolation medium for fastidious organisms. The peptones included have been chosen for maximum growth stimulation. Starch and sodium bicarbonate act as de-toxification agents while hemin encourages pigment production in Porphyromonas melaninogenicus. Specific growth promoting agents are Cysteine for Fusobacterium necrophorum, Propionibacterium acne and Bacteriodes fragilis, arginine for Eubacterium spp. soluble pyrophosphate for Porph. gingivalis and Porph. asaccharolyticus.For more information visit https://www.neogen.com/solutions/Microbiology/fastidious-anaerobe-broth
NEOGEN Sabouraud Dextrose Agar with Lecithin & Tween, 500 g
Sabouraud Dextrose Agar (with Lecithin & Tween) is used for the isolation of fungi from surfaces sanitized with quaternary ammonium compounds in a laboratory setting. Sabouraud Dextrose Agar (with Lecithin & Tween) is not intended for use in the diagnosis of disease or other conditions in humans. Sabouraud Dextrose Agar (SDA) is a modification of Dextrose Agar described by Sabouraud. SDA is used for cultivating pathogenic and commensal fungi and yeasts. The high dextrose concentration and acidic pH of the formula permits selectivity of fungi. The addition of Lecithin and Tween to Sabouraud Dextrose Agar is used to neutralize antiseptics and disinfectants for environmental monitoring and other applications. Complete neutralization of disinfectants is important. Disinfectant carryover can cause false no-growth test results.For more information visit https://www.neogen.com/solutions/Microbiology/sabouraud-dextrose-agar-with-lecithin-and-tween
NEOGEN Dermatophyte Test Medium (DTM), 500 g
Dermatophyte Test Medium is used for the selective isolation of dermatophytic fungi and is not intended for use in the diagnosis of disease or other conditions in humans.In 1969, Taplin et al. developed this medium for the isolation and recognition of dermatophytic fungi, the causative agent of ringworm from hair, nails, and skin. Dermatophyte Test Medium is preferred for isolation and early recognition of Microsporum, Trichophyton, and Epidermophyton genera because of a distinct color change in the medium. Rapidly-growing species may produce a complete color change in the medium in 3 days. The slower-growing species will change the indicator in longer time periods. Other organisms may grow but can be recognized as non-dermatophytes by lack of a color change.For more information visit https://www.neogen.com/solutions/Microbiology/dermatophyte-test-medium-dtm
NEOGEN Bile Esculin Azide Agar, 10 kg
Bile Esculin Azide Agar is used for the selective isolation and differentiation of group D streptococci in a laboratory setting. Bile Esculin Azide Agar is not intended for use in the diagnosis of disease or other conditions in humans.Bile Esculin Azide Agar is a modification of the medium reported by Isenberg and Isenberg, Goldberg, and Sampson. This formula modifies Bile Esculin Agar by adding sodium azide and reducing the concentration of bile. The revised medium is more selective, but still provides rapid growth and efficient recovery of group D streptococci.Molecular taxonomic studies of the genus Streptococcus have placed enterococci, previously described group D streptococci, in the genus Enterococcus. The ability to hydrolyze esculin in the presence of bile is a characteristic of enterococci and group D streptococci.For more information visit https://www.neogen.com/solutions/Microbiology/bile-esculin-azide-agar
NEOGEN WL Nutrient Agar, 500 g
W-L Nutrient Agar is used for the cultivation of yeasts, molds, and bacteria encountered in brewing and industrial fermentations. W-L Nutrient Agar is not intended for use in the diagnosis of disease or other conditions in humans. W-L Nutrient Medium was developed by Green and Gray while studying various fermentation processes. An exhaustive study examining methods of fermentation control procedures in worts, beers, liquid yeasts and similar fermentation products led to the development of W-L Nutrient Medium. At a pH of 5.5, counts of viable baker’s yeast will grow on W-L Nutrient Medium. W-L Nutrient Medium is also referred to as “Wallerstein Laboratory Medium”.For more information visit https://www.neogen.com/solutions/Microbiology/wl-nutrient-agar
NEOGEN Xylose Lysine Deoxycholate (XLD) Agar (ISO), 500 g
This item has a minimum order quantity of 2 per supplier requirements.
Xylose Lysine Deoxycholate (XLD) Agar (ISO) is a selective agar for the detection of Salmonella spp. in food, animal feed and in environmental samples from the food production area as described in ISO 6579-1:2017. Xylose Lysine Deoxycholate (XLD) Agar (ISO) is not intended for use in the diagnosis of disease or other conditions in humans. A selective, diagnostic agar for the detection of Salmonella spp. in food, animal feed and in environmental samples from the food production area as described in ISO 6579-1:2017. Originally formulated by Taylor to differentiate enteric pathogens, the agar is widely used as the preferred differential medium for Salmonella spp .For more information visit https://www.neogen.com/solutions/Microbiology/xylose-lysine-deoxycholate-xld-agar-iso
NEOGEN Columbia Agar Base, 10 kg
Columbia Agar Base is used with blood for the isolation and cultivation of a wide variety of fastidious microorganisms. Columbia Agar Base is not intended for use in the diagnosis of disease or other conditions in humans. A modification of the original Columbia Agar base formulation, Columbia Agar Base provides a medium that is suitable for use with both defibrinated horse and sheep blood. The peptone mixture provides a source of nitrogen, essential vitamins and amino acids. The starch provides a carbon source and sodium chloride maintains osmotic balance. Originally described as a general purpose nutritious agar base by Ellner et al. Columbia Agar is more frequently used when enriched by the addition of sterile blood. The medium is suitable for supporting the growth and determining hemolytic reactions of a variety of microorganisms For more information visit https://www.neogen.com/solutions/Microbiology/columbia-agar-base